Kingpin (Wilson Fisk) Created by writer Stan Lee and artist John Romita Sr., Kingpin first appeared in The Amazing Spider-Man #50 (July 1967).
Colonel Nicholas Joseph "Nick" Fury Created by artist Jack Kirby and writer Stan Lee, Nick Fury first appeared in Sgt. Fury and his Howling Commandos #1 (May 1963).
She-Hulk (Jennifer Susan Walters) Created by writer Stan Lee and artist John Buscema, she first appeared in Savage She-Hulk #1 (February 1980).
Blade (Eric Brooks) Created by writer Marv Wolfman and penciller Gene Colan, Blade first appeared in The Tomb of Dracula #10 (July 1973).
Mary Jane Watson Created by writer Stan Lee and artist John Romita, Sr., after a few partial appearances and references, Mary Jane Watson finally first fully appeared in The Amazing Spider-Man #42 (November 1966).
Galactus (Galan) Created by writer-editor Stan Lee and artist and co-plotter Jack Kirby, Galactus first appears in Fantastic Four #48 (March 1966).
Nightcrawler (Kurt Wagner) Created by writer Len Wein and artist Dave Cockrum, Nightcrawler first debuted in Giant-Size X-Men #1 (May 1975).
Black Widow (Natalia Alianovna Romanova) Created by Stan Lee, Don Rico, and Don Heck, Black Widow first appeared in Tales of Supsense #52 (April 1964)
Doctor Octopus (Dr. Otto Gunther Octavius) Created by Stan Lee, Doctor Octopus first appeared in Amazing Spider-Man #3 (July 1963)
Storm (Ororo Iqadi T'Challa, née Munroe) Created by Len Wein and Dave Cockrum, Storm first appeared in Giant-Size X-Men #1 (May 1975)